Measuring insulin

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Measuring insulin

ancreatic ␤ cells that exocytose insulin must make more insulin. Trajkovski et al., on page 1063, now demonstrate that the signal to induce insulin synthesis after exocytosis comes from the cleavage of the cy-toplasmic domain of islet cell autoantigen 512 (ICA512). This protein fragment migrates to the nucleus and drives changes in gene expression. Previous work showed that ICA512 is a transmem...

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Measuring β-Cell Function Relative to Insulin Sensitivity in Youth

OBJECTIVE To compare β-cell function relative to insulin sensitivity, disposition index (DI), calculated from two clamps (2cDI, insulin sensitivity from the hyperinsulinemic-euglycemic clamp and first-phase insulin from the hyperglycemic clamp) with the DI calculated from the hyperglycemic clamp alone (hcDI). RESEARCH DESIGN AND METHODS Complete data from hyperglycemic and hyperinsulinemic-eu...

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Fishing in the stream of diabetes: from measuring insulin to the control of fetal organogenesis.

The measurement of insulin Non-insulin-dependent diabetes mellitus (NIDDM) is estimated to affect 100 million people worldwide. It has been observed to increase in prevalence when a population increases its plane of nutrition and decreases its energy expenditure. A striking example of this occurred when Ethiopian Jews were moved to Israel [l]. The Pacific population of the island of Nauru was d...

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Critical variables in the radioimmunological technique for measuring immunoreactive insulin with use of immunosorbents.

A commercial kit for determining immunoreactive insulin, the “Phadebas Insulin Test,” was methodically evaluated to establish the optimal conditions for this immunoassay. Sensitivity, day-to-day and withinrun precision, recovery, accuracy, and specificity are excellent. Results by this method compare favorably with those of the double-antibody technique. This rapid, simple, convenient, and reli...

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Measuring b-Cell Function Relative to Insulin Sensitivity in Youth Does the hyperglycemic clamp suffice?

RESEARCH DESIGN AND METHODSdComplete data from hyperglycemic and hyperinsulinemic-euglycemic clamps were available for 330 youth, including 73 normal weight (NW), 168 obese with normal glucose tolerance (OB-NGT), 57 obese with impaired glucose tolerance (OB-IGT), and 32 obese with type 2 diabetes (OB-T2DM). The correlation between hcDI and 2cDI and Bland-Altman analysis of agreement between the...

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ژورنال

عنوان ژورنال: Journal of Cell Biology

سال: 2004

ISSN: 1540-8140,0021-9525

DOI: 10.1083/jcb1676iti1